Experimentally manipulated biota over a 30-40d period in two streams with distinctly different macrobiotic assemblages

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Here we test the hypothesis that differences in macrobiotic assemblages can lead to differences in the quantity and quality of organic matter in benthic depositional environments among streams in montane Puerto Rico. We experimentally manipulated biota over a 30-40d period in two streams with distinctly different macrobiotic assemblages: one characterized by high densities of omnivorous shrimps (Decapoda: Atyidae and Xiphocarididae) and no predaceous fishes. To incorporate the natural hydrologic regime and to avoid confounding artifacts associated with cage enclosure/exclosure (e.g., high sedimentation), we used electricity as a mechanism for experimental exclusion, in situ. In each stream, shrimps and/or fishes were excluded from specific areas of rock substrata in four pools using electric "fences" attached to solar-powered fence chargers. In the stream lacking predaceous fishes (Sonadora), the unelectrified control treatment was almost exclusively dominated by high densities of omnivorous shrimps that constantly ingested fine particulate material from rock surfaces. Consequently, the control had significantly lower levels of inorganic sediments, organic material, carbon and nitrogen than the exclusion treatment, as well as less variability in these parameters. Tenfold more organic material (as ash-free dry mass, AFDM) and fivefold more nitrogen accrued in shrimp exclosures (10.6 g AFDM/m2, 0.2 g N/m2) than in controls (1.1 g AFDM/m2, 0.04 g N/m2). By reducing th quantity of fine particulate organic material and associated nitrogen in benthic environments, omnivorous shrimps potentially affect the the supply of this important resource to other trophic levels. The small amount of fine particulate organic matter (FPOM) that remained in control treatments (composed of sparse algal cells0 was of higher quality than that in shrimp exclosures. This is evidenced by the significantly lower carbon-to-nitrogen (C/N) ratio (an indicator of food quality, with relatively low C/N indicating higher food quality) in the control relative to the shrimp exclosure treatment. In contrast, the stream with predaceous fishes (Bisley) was characterized by very low numbers of shrimps, and macrobiota had no significant effect on benthic sediments, organic matter, C, N, and C/N. All parameters were highly variable through time, with levels and ranges in variability similar to the shrimp exclusion treatment in the Sonadora. Our experimental results are consistent with findings of an independent survey of six streams in four different drainages. Four streams that had an abundance of omnivorous shrimps but lacked predaceous fishes, had extremely low levels of fine benthic organic and inorganic material. In contrast, two streams that had low densities of shrimps and contained predaceous fishes had significantly higher levels. Results show a strong linkage between species and ecosystem characteristics: interstream differences in the quantity and quality of fine benthic organic matter resources were determined by the nature of the macrobiotic assemblage. Furthermore, patterns in the distribution of shrimp assemblages reflected landscape patterns in the benthic depositional environment among streams.

Date Range: 
1994-06-20 00:00:00 to 1994-08-13 00:00:00

Publication Date: 

2011-04-04 00:00:00



Additional Project roles: 

Name: Eda Melendez-Colom Role: Data Manager
Name: Angel Bednarek Role: Associated Researcher
Name: James G. March Role: Associated Researcher
Name: Nina Hemphill Role: Associated Researcher
Name: William H. McDowell Role: Associated Researcher


Biotic manipulations were conducted in the Sonadora and 30 d in the Bisley - 3 stream. Experiments were staggered to accomodate other studies being conducted in these gauged watersheds. In both streams we used circular electrified "fences" (hoops) to excludes fishes and shrimps larger than 1 cm in length from foraging within specific areas of the stream bottom. For a more detailed description of the methodology see publication .



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