Bisley Watershed 3 and Quebrada Prieta Algae Monitoring

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The LTER is conducting annual monitoring of Algae, Chlorophyll a, benthic organic matter, and benthic inorganic matter in order to document baseline stream characteristics. These data may be used to examine effects of disturbances such as hurricanes on stream ecosystems.

Date Range: 
2003-06-01 00:00:00 to 2016-07-31 00:00:00



Additional Project roles: 

Name: Eda Melendez-Colom Role: Data Manager
Name: Effie A. Greathouse Role: Associated Researcher
Name: Katherine Smith Role: Associated Researcher


Field Methods: We sampled 5 pools and 5 riffles in each stream. In each pool and in each riffle, we took 6 haphazardly chosen epilithic samples using a suction device modified from Loeb (1981, An in situ method for measuring the primary productivity and standing crop of the epilithic periphyton community in lentic systems. Limnology and Oceanography 26:394-399). The 6 samples were composited into a nalgene bottle for later analysis. We thus had a single composited sample for each pool and each riffle. Bottles were brought back to the laboratory on ice. Data were collected twice per year (Spring~January and Summer ~June to August) from January 2002 to present. Loeb samples in pools were from rocks/boulders under water that was at least 3 inches deep. No specific depth criteria was used for riffles because they are shallow, but the sampled spot had to be deep enough for the Loeb sampler to work (i.e. the water had to be as deep as the tiny side hole of the Loeb sampler). Riffle Loeb samples were only taken where the water was actually flowing.

Lab methods: Each sample was sub-sampled for filtration onto 2 glass fiber filters. The first filter was a pre-weighed, pre-ashed filter for AFDM/inorganic matter analysis. The second filter was a pre-ashed filter for chlorophyll a analysis. Volumes filtered onto each filter were recorded as well as any volume not filtered. Three drops of 1% MgCO3 were added to the chlorophyll subsample during filtration. Chlorophyll filters were immediately frozen. AFDM/inorganic matter filters were immediately dried at 50 C for at least 24 hours. Later, chlorophyll filters were analyzed fluorometrically according to standard methods [APHA (American Public Health Association). 1985. Standard methods for the examination of water and wastewater, 16. American Public Health Association, Washington, D.C.]. The AFDM/inorganic matter filter was later weighed to the nearest 0.001 g, ashed at 500 C for 3 hours, and re-weighed to determine inorganic and ash-free dry mass (AFDM) per unit area.



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